Multiscale photoacoustic microscopy and computed tomography.

Prospects of photoacoustic tomography.

Commercially available high-resolution three-dimensional optical imaging modalities-including confocal microscopy, two-photon microscopy, and optical coherence tomography-have fundamentally impacted biomedicine. Unfortunately, such tools cannot penetrate biological tissue deeper than the optical transport mean free path (approximately 1 mm in the skin). Photoacoustic tomography, which combines ...

Multiscale photoacoustic microscopy of single-walled carbon nanotube-incorporated tissue engineering scaffolds.

Three-dimensional polymeric scaffolds provide structural support and function as substrates for cells and bioactive molecules necessary for tissue regeneration. Noninvasive real-time imaging of scaffolds and/or the process of tissue formation within the scaffold remains a challenge. Microcomputed tomography, the widely used technique to characterize polymeric scaffolds, shows poor contrast for ...

PACM: Photoacoustic Computed Microscopy

Photoacoustic Computed Microscopy (PACM) is a new label-free microscopic method that combines current photoacoustic microscopy technique with a modelbased inverse reconstruction algorithm to provide functional images of microvasculature. This article demonstrates its in vivo imaging ability of quantifying important functional parameters at the small vessel level in a rodent model. This new tech...

Photoacoustic Microscopy and Photoacoustic Computed Tomography Using High-frequency Linear Array Ultrasonic Transducers

Photoacoustic computed microscopy

Photoacoustic microscopy (PAM) is emerging as a powerful technique for imaging microvasculature at depths beyond the ~1 mm depth limit associated with confocal microscopy, two-photon microscopy and optical coherence tomography. PAM, however, is currently qualitative in nature and cannot quantitatively measure important functional parameters including oxyhemoglobin (HbO2), deoxyhemoglobin (HbR),...